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g5 strain

Putative nuclease G5

Annotation score:2 out of 5

The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.

– Experimental evidence at transcript level i

This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

Select a section on the left to see content.

This section provides any useful information about the protein, mostly biological knowledge.

This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.

Sites

This subsection of the Function section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.

The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

GO – Molecular function i

  • metal ion binding Source: UniProtKB-KW
  • nuclease activity Source: UniProtKB-KW
GO – Biological process i
  • DNA repair Source: UniProtKB-KW

UniProtKB Keywords constitute a controlled vocabulary with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.

Molecular function Hydrolase, Nuclease
Biological process DNA damage, DNA repair
Ligand Magnesium, Metal-binding

This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

Names & Taxonomy i

This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.

This subsection of the Names and taxonomy section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.

This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.

This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.

This subsection of the Names and taxonomy section only exists in viral entries and indicates the host(s) either as a specific organism or taxonomic group of organisms that are susceptible to be infected by a virus.

This subsection of the Names and taxonomy section is present for entries that are part of a proteome, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

A UniProt proteome can consist of several components.
The component name refers to the genomic component encoding a set of proteins.

This section provides information on the location and the topology of the mature protein in the cell.

Subcellular location i

  • Virion By similarity Note: Present in the virion core. By similarity
GO – Cellular component i
  • virion Source: UniProtKB-SubCell
Keywords – Cellular component i

This section describes post-translational modifications (PTMs) and/or processing events.

PTM / Processing i

Molecule processing

This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.

Proteomic databases

PRoteomics IDEntifications database

This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.

This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).

Keywords – Developmental stage i

This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

Family & Domains i

This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.

Sequence similarities i

Family and domain databases

Integrated resource of protein families, domains and functional sites

Pfam protein domain database

This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight. The information is filed in different subsections. The current subsections and their content are listed below:

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

Sequence status i : Complete.

The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.

It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.

However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x 64 + x 4 + x 3 + x + 1. The algorithm is described in the ISO 3309 standard.

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
Cyclic redundancy and other checksums
Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

Checksum: i D1DD4DC56A0AF5A0

Sequence databases

EMBL nucleotide sequence database

GenBank nucleotide sequence database

DNA Data Bank of Japan; a nucleotide sequence database

Protein sequence database of the Protein Information Resource

This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

Similar proteins i

  • 100% Identity
  • 90% Identity
  • 50% Identity

This section is used to point to information related to entries and found in data collections other than UniProtKB.

Sequence databases
3D structure databases

Database of comparative protein structure models

SWISS-MODEL Interactive Workspace

Proteomic databases
Family and domain databases

ProtoNet; Automatic hierarchical classification of proteins

MobiDB: a database of protein disorder and mobility annotations

This section provides general information on the entry.

Entry information i

This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.

This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.

This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the canonical sequence are also displayed.

This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).

This section contains any relevant information that doesn’t fit in any other defined sections

Putative nuclease that seems to be required for double-strand break repair, homologous recombination, and production of full-length viral genomic DNA.